Alix Sausset

Member of the IMI team (Imaging & Infectiology), expert in flow cytometry activities and head of sample preparation for Single Cell approaches

Research

I perform cell analyses and sorting using flow cytometry on a wide range of samples, utilizing a high-speed cell sorter (MoFlo Astrios EQ, BeckmanCoulter). Cells purified through cell sorting are directly applicable in various methodologies (transcriptomic analyses, proteomic analyses, microscopy, experimental infections, etc.).
I am responsible for two pieces of equipment:
> a cell analyzer (LSR-FortessaTM X-20, Becton-Dickinson) available for self-service use after completing training sessions that I provide.
> an apparatus used for preparing samples for single-cell transcriptomic analyses (Chromium Controller, 10X Genomics). I train new users in operating this equipment and coordinate all Single Cell experiments, assisting users in organizing their experiments.

Fields of expertise: Cytometry, high-speed cell sorting, microtiter plate sorting and cloning, pathogen sorting (bacteria, parasites), cytometry software (Kaluza, Summit, FACSDiva...), fluorescence, Single Cell.

Contact details

Alix Sausset

  Alix Sausset
  INRAE Val de Loire
  UMR ISP - 311
  37380 Nouzilly
  France
  33(0)2 47 42 78 74
  Email : alix.sausset@inrae.fr

 

Publications

HAL
Pubmed

See also

Modification date : 18 December 2023 | Publication date : 31 August 2023 | Redactor : Alix Sausset